The Islamic Republic of Iran Broadcasting (IRIB) building was struck in an Israeli attack on Monday, cutting live coverage immediately.
The blast occurred as the presenter was live on TV lambasting Israel before she was seen leaving the live broadcast, Iranian media reported, sharing a video of the incident.
An Iranian missile struck an oil refinery in Israel's coast city of Haifa on Sunday, killing three people, an Israeli official said after a gag order was lifted.
The confirmation on Monday removed reporting restrictions imposed by Israel's military censorship on the attack, part of the most intense confrontation in history between longtime foes Iran and Israel.
Thick black smoke could be seen rising from the the sprawling complex early Monday, according to an AFP journalist.
Iran has been launching nightly barrages targeting Israel, with some missiles evading the country's sophisticated air defence batteries.
The Iranian attacks have hit the central hub of Tel Aviv and other cities and towns including Haifa, destroying buildings, causing fires and leaving residents stunned and picking through the rubble.
Israeli officials said 11 people were killed by Iranian missiles overnight, taking the overall death toll since the attacks began on Friday to 24.
In Iran, the health ministry said at least 224 people had been killed.

Smoke billows from an explosion in southwest Tehran on Monday. AFP

This combo of image grabs taken from footage broadcast by Iran's IRINN news on Monday shows (clockwise from left) a news reporter gesturing as she presents the news, her reaction after a loud noise, her leaving the room and her chair and microphone engulfed by smoke. IRIN /AFP

Smoke rises from a refinery's compound in Haifa, Israel, on Monday, in this screengrab taken from a video obtained by Reuters.